Bacterial Multidrug Exporters by Akihito Yamaguchi & Kunihiko Nishino

Author:Akihito Yamaguchi & Kunihiko Nishino
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

4.The purified His-tagged AcrB was eluted by applying 100 μL of 500 mM imidazole elution buffer to the matrix and allowed to sit at room temperature for 5 min and centrifuged at 6000 × g for 1 min.

3.5 Gel Separation and Image Capture for Analysis

1.10 μL of the purified protein was resolved in 11% SDS protein gel.

2.The gel was separated from the plate and gently washed with water.

3.An image of gel was captured with Typhoon FLA 9500 with filters set at excitation at 488 nm and emission at 510 nm.

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